| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1091 | Holdemania filiformis | CTGATCGGACAGTGGCGTT | TTTGGCAATCGCGATCGC | 60.08 | 59.60 | 240 | 45 |
100.00%
|
50.00%
|
| Primer 1092 | Holdemania filiformis | ATGTCGGCAGCGGAGTAA | CGCGACGTCAAACTTGCA | 58.71 | 59.38 | 161 | 45 |
100.00%
|
50.00%
|
| Primer 1093 | Holdemania filiformis | TCCAGCAGGCGTGTGAAA | TCGCGATGATCGGCATGT | 59.49 | 59.58 | 270 | 45 |
100.00%
|
50.00%
|
| Primer 1094 | Holdemania filiformis | TGCTGACCCTGCATCGTT | AACGTCGCCAGATCGCTT | 59.25 | 59.74 | 160 | 45 |
100.00%
|
50.00%
|
| Primer 1095 | Holdemania filiformis | GCGGTTTCTGAACGCGTT | ACGATGGCATTGCGCAGA | 59.37 | 60.44 | 171 | 45 |
100.00%
|
50.00%
|
| Primer 1096 | Holdemania filiformis | GCGGTTTCTGAACGCGTT | AGAGCGCTGATGCACGAT | 59.37 | 59.50 | 184 | 45 |
100.00%
|
50.00%
|
| Primer 1097 | Holdemania filiformis | ACGCGTCGCTGAGCTTAA | AGTCGTGAAGCTGCCGTT | 59.74 | 59.58 | 299 | 45 |
100.00%
|
50.00%
|
| Primer 1098 | Holdemania filiformis | ACGGCAATGCTTCCAGGTT | ATCGTGTTGCCGTCAGGT | 60.23 | 59.26 | 182 | 45 |
100.00%
|
50.00%
|
| Primer 1099 | Holdemania filiformis | GTGGAAACGCTCAAAGCCG | ATGGCAGCAGCGTTTCCT | 60.08 | 59.97 | 253 | 45 |
100.00%
|
50.00%
|
| Primer 1100 | Holdemania filiformis | TTCCAGTTCGCTGCCGTT | TGCGGATCAACGGCATGA | 59.89 | 59.73 | 188 | 45 |
100.00%
|
50.00%
|